################################################################### # Additional strains sequenced at MPI by collaborators # date: 2011_06_28 ################################################################### The three strains (Ws-2, Tnz-1 and Strand-1) were sequenced by Seth Davis (Max Planck Institute for Plant Breeding Research, Cologne) and were analyzed at the MPI Tuebingen. ################################################################### # Folder organisation ################################################################### Within the folder 'strains' there are subfolders for each strain containing analyzed data files. As the short read analysis was performed against TAIR8, the directory structure looks like: `-- strains `-- `-- TAIR8 The analyzed data files are: filtered_variant.txt Positions and annotation of SNPs (single nucleotide polymorphisms) and 1-3bp deletions detected through short read alignments, including a quality value. filtered_reference.txt Positions featuring the reference base, including a quality value. SV_deletion_high_quality.PE.txt Large deletion prediction. Note deletions shorter than 10bp have a bad selectivity and sensitivity and have only been included for completeness. An unique ID value was attached to each prediction in order to find the identical deletion in other accessions again. This allows for frequency analysis of SV deletions. unsequenced.txt Describes all regions which did not allow for a base call (including SV deletions) independent of the reason. ################################################################### # File format description ################################################################### filtered_variant.txt <# of nonrepetitive reads supporting substituion> filtered_reference.txt <# of nonrepetitive reads supporting substituion> SV_deletion_high_quality.PE.txt <# read pairs supporting SV call> <# positions w/o nonrepetitive core alignment> <# positions w/o core alignment> unsequenced.txt ------------------------------------------------------------------- Tuebingen, Germany, 2011 Questions? Seth Davis Jun Cao